FIG. 4.

Western analysis demonstrating the presence of CRP in tissue culture supernatant from Detroit 562 pharyngeal carcinoma cells. (A) Lane 1, purified human CRP (2 ng); lane 2, tissue culture medium from Detroit 562 pharyngeal carcinoma cells (5 μl); lane 3, tissue culture medium alone (5 μl). (B) Equivalent amounts tissue culture supernatant from Detroit 562 pharyngeal carcinoma cells treated (lane 1) or untreated (lane 2) with ChoP-agarose beads which bind CRP. Lane 3, purified CRP (4 ng). Sizes are indicated in kilodaltons.