TABLE 3.
Percentage of CD3+ splenocytes expressing different intracellular cytokines with or without in vitro stimulationa
Stimulation | % of cytokine-expressing cells from mice immunized withb:
|
|||||||
---|---|---|---|---|---|---|---|---|
KETc1
|
KETc12
|
|||||||
IL-2 | IL-4 | IL-10 | INF-γ | IL-2 | IL-4 | IL-10 | INF-γ | |
None (media) | 1.4 | 0.7 | 0.6 | 0.6 | 1.6 | 0.9 | 1.2 | 0.7 |
Peptide | 8.1 | 4.4 | 3.0 | 11.2 | 9.4 | 3.2 | 4.8 | 14.5 |
Cysticercal antigens | 13.0 | 4.0 | 3.6 | 6.7 | 16.2 | 2.6 | 4.2 | 17.0 |
Pooled splenocytes from three KETc1 or KETc12 immunized mice were analyzed for intracellular cytokines 60 h after in vitro stimulation.
The percentages of CD3+ cells expressing the four different interleukins (not included) were below 1% in splenocytes from control mice with and without in vitro stimulation. Data are representative of two different experiments using different mice.