Figure 2.

Connective tissue phenotype mast cells (MC_TCs) disrupt endothelial cell tube formation. A: Representative, color-enhanced images of fetal human pulmonary microvascular endothelial cells (feHPMVCs) undergoing tube morphogenesis when cultured for 12 hours alone (blue) and with MC_TCs (red) or mucosal phenotype mast cells (MC_Ts) (green). B: Quantification of tube complexity (Materials and Methods) defined each hour during tube formation. C: Graphical representation of the mean time (in hours) needed to reach maximum tube structure complexity for each condition. D: Graphical representation of the mean time (in hours) needed for tube structures to regress to 70% of their maximum complexity. n = 3 (and in triplicates). ∗∗∗∗P < 0.0001 control versus MC_TC co-culture (χ² test).