Figure 4.

Connective tissue phenotype mast cells (MC_TCs) destabilize endothelial cell-cell junctions. A: Quantification of paracellular permeability of fetal human pulmonary microvascular endothelial cell (feHPMVC) monolayer alone (control; blue) and with MC_TCs (red) or mucosal phenotype mast cells (MC_Ts; green). B: Quantification of transendothelial electrical resistance (TEER) in confluent cultures of feHPMVC monolayers alone (control) (blue) and with MC_TCs (red) or MC_Ts (green). C: Representative images of feHPMVC monolayers in each condition indicated. Cell-cell contacts are visualized by staining for CDH5 (green), and endothelial cell surface glycoprotein (UEA1) and nuclei are visualized by DAPI. n = 4 (and in triplicates). ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 (t-test). Scale bars = 100 μm.