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. 2023 Jan;29(1):30–43. doi: 10.1261/rna.079472.122

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© 2023 Yu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available undera Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 1. — TCFL5 is expressed in pachytene spermatocytes during male meiosis I. (A,B) A-Myb and Tcfl5 mRNA (A) and protein abundance (B) at distinct developmental stages of mouse spermatogenesis. See Supplemental Figure S1A for uncropped western blot images. (C) Abundance of FLAG-TCFL5 protein in FACS-purified germ cell from Tcfl5^+/FLAG mouse. Each lane contained protein from 75,000 germ cells. (D) mRNA abundance of Tcfl5 mRNA measured by single-cell sequencing by Green et al. (2018). (E) Immunohistochemical detection of A-MYB in adult testis section. A-Myb^−/− testis sections serve as antibody specificity controls. (F) Immunohistochemical detection of TCFL5 in testis sections from staged mice. Tcfl5^em1/em1 testis sections serve as antibody specificity controls. (Spg) Spermatogonia, (SpI) primary spermatocytes, (SpII) secondary spermatocytes, (RS) round spermatids, (ES) elongating spermatids, (SC) Sertoli cells.