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. 2023 Jan;29(1):30–43. doi: 10.1261/rna.079472.122

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© 2023 Yu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available undera Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 4. — The role of TCFL5 in piRNA production is conserved in rhesus macaque. (A, left) Percent identity of mouse TCFL5 to TCFL5 in other mammals. (Right) Unrooted tree of TCFL5 protein sequences, aligned using Clustal Omega. The unrooted tree was constructed using Randomized Axelerated Maximum Likelihood with default parameters and visualized in the Interactive Tree Of Life. (B) A-MYB and TCFL5 ChIP-seq peaks at the promoters of the rhesus macaque A-MYB and TCFL5 genes. (C) The distance from the nearest TCFL5 ChIP-seq peak to the transcription start site (TSS) for rhesus genes separated by class. Vertical lines: median; whiskers: maximum and minimum values, excluding outliers. A single marker containing the number of data points marks multiple identical values.