FIG. 3.

Massive infiltration of neutrophils leading to microabscess formation following Listeria infection in Csf1^op/Csf1^op mice. (a through d) Liver sections from 24- and 48-h-postinfected mice were immunostained with RB6-8C5 antibody to stain neutrophils in tissues. There were fewer neutrophils in +/Csf1^op mice (a) compared to the massive accumulation of neutrophils seen in Csf1^op/Csf1^op mice (b). Staining at higher magnification is shown (c and d) with arrowheads indicating RB6-8C5⁺ neutrophils in +/Csf1^op mice (c) and a typical micro abscess filled with RB6-8C5⁺ cells in Csf1^op/Csf1^op mice (d). Data shown are from 24 h p.i. Similar results were obtained at 48 h p.i.