Nitric oxide (NO) is a gaseous mediator to maintain vascular homeostasis. A hallmark of endothelial dysfunction and subsequent pro-thrombotic events is endothelial NO synthase (eNOS) suppression with concomitant NO deficiency, resulting in vasoconstriction, inflammation, platelet activation, hypercoagulation, and cardiovascular diseases. Photobiomodulation (PBM) with near-infrared (NIR) light exposure has the potential to increase NO bioavailability via multiple mechanisms.