Fig. 1.

Effect of NBP on MPP + -induced cytotoxicity of N2A cells. (A) Cells were stimulated with NBP at a concentration of 0, 0.1, 0.5, 1, 5 or 10 μM for 24 h, and the cell viability was determined by MTT assay. (B) Different concentrations of MPP⁺ (0, 40, 100, 250, 500 and 1000 μM) was added to co-cultured with N2A cells for 24 h, and the cell viability was assayed with MTT. (C) Cells were pretreated with different concentrations of NBP (0, 0.1, 0.5, 1, 5 or 10 μM) for 24 h, and then these cells were subjected to MPP⁺ (500 μM) insults