Table 3. The real-time PCR conditions for mRNAs and miRNAs .
| Bio-materials | Temperature (C°) | Duration | Cycles |
| mRNAs | 95 | 5 min | 1 |
| 95 | 10 s | ||
| Tm | 35 s | 40 | |
| 72 | 20 s | ||
| miRNAs | 95 | 2 min | 1 |
| 95 | 5 s | 40 | |
| 60 | 30 s |
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