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. Author manuscript; available in PMC: 2023 Jan 3.
Published in final edited form as: Methods Mol Biol. 2015;1251:25–42. doi: 10.1007/978-1-4939-2080-8_2

Fig. 5.

Fig. 5

Calcium imaging of neuronal ensemble activity with 2PE microscopy. (a) Typical field of view of layer 2/3 neurons (green) and glia (yellow) stained with the calcium indicator dye Oregon Green BAPTA-1AM and imaged with in vivo 2PE microscopy. Sulforhodamine 101 (a red dye) was used to stain glia. The image is a single frame (~120 μm below the dura) in a representative calcium imaging movie (3 min, 3.9 frames per second) from a 14-day-old mouse. (b) Calcium traces showing the relative changes in fluorescence intensity over the baseline fluorescence (ΔF/F) of 5 different layer 2/3 neurons from a representative calcium movie. The upward deflections represent spiking events within those neurons