Figure 7.

Oxygen consumption rates of differentiated ADSCs with conditioned medium of IPI-549 treated and untreated SVF of lipedema and control patients. (A) Evaluation of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) changes in response to mitochondrial respiratory function stress test using sequential applications of A = oligomycin, B = FCCP, C = Antimycin A & Rotenone (B) No significant alterations were observed in mitochondrial respiration, maximal respiration, ATP-linked respiration nor non-mitochondrial respiration, (C) Evaluation of the metabolic phenotype showed alterations of the ECAR baseline and ECAR metabolic potential of ADSCs differentiated with lipedema conditioned medium versus control. The treatment with IPI-549 normalized the lipedema ECAR metabolic potential to the control level. N(C) = 5, N(L) = 5. For the comparison between the treated and untreated sample of the same patient a paired T-test was used, for the comparison of the untreated lipedema to untreated control an unpaired T-test was used. OCR and ECAR values are normalized to cell numbers. *P < 0.05; **P < 0.01.