TABLE 1.
Entry of IH11128 bacteria into undifferentiated or differentiated nonintestinal and intestinal epithelial cells expressing CD55a
| Cell | Expression amount and pattern of CD55b | Mean level of bacteria (CFU/ml) ± SEMc
|
|
|---|---|---|---|
| Cell associated | Intracellular | ||
| HeLa (N, U) | 1.35 ± 0.15; diffuse labeling | 1.81 × 107 ± 0.14 × 107 | 1.26 × 105 ± 0.14 × 105 (0.696) |
| INT407 (I, U) | 1.40 ± 0.24; punctate labeling | 2.77 × 107 ± 0.88 × 107 | 3.10 × 104 ± 0.87 × 104 (0.112) |
| Caco-2/TC7 cells at day 6 in culture (I, U) | 1.38 ± 0.18; punctate labeling | 2.10 × 107 ± 0.86 × 107 | 3.23 × 104 ± 0.74 × 104 (0.154) |
| Caco-2/TC7 cells at day 15 in culture (I, D) | 1.40 ± 0.18; punctate labeling with mosaic pattern | 2.35 × 107 ± 0.75 × 107 | 8.86 × 102 ± 0.67 × 102 (0.0037) |
Cells were infected with IH11128 bacteria for 3 h at a concentration of 108 CFU/ml. N, nonintestinal cells; I, intestinal cells; U, undifferentiated cells; D, differentiated cells.
Relative immunofluorescence intensity was measured with a conventional fluorescence microscope connected to a Visiolab 1000 image analyzer. Results are expressed as arbitrary units.
The levels of cell-associated bacteria were determined after the cells were lysed with H2O. The levels of intracellular bacteria were determined by gentamicin assay. The values in parentheses are the percentages of cell-associated bacteria that were also intracellular.