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. 2022 Nov 21;13(15):3640–3651. doi: 10.7150/jca.77238

Figure 3.

Figure 3

Knockdown of KIF4A suppresses glioma cell proliferation and cell cycle in vitro. (A, B) KIF4A expression at the protein level in U87 and U251 glioma cells was evaluated by Western blot after transfection. (C, D) KIF4A knockdown inhibits the capacity of colony formation in U251 and U87 glioma cells compared with negative transfected cells (NC). (E, F) CCK-8 assays revealed that silence of KIF4A suppresses (SH) cell proliferation of U251 and U87 glioma cells compared with negative transfected cells (NC). (E, F) Knockdown of KIF4A increased G0/G1 phase cell population, as detected by flow cytometric analysis following Annexin FITC and PI staining. Data are shown as mean ± SD. * p<0.05; ** p<0.01; *** p<0.001.