TABLE 1.
Reactivity of sera against synthetic peptides modelled on defined epitopesa
| Immunizing peptide | Reactivity with testing peptide
|
|||||||
|---|---|---|---|---|---|---|---|---|
| 1439 | 1441 | 1443 | 1 | 2 | 3 | 4 | 5 | |
| Mouse sera | ||||||||
| 1439 | ++ | − | − | − | − | − | ± | − |
| 1441 | + | ++ | − | + | + | + | + | − |
| 1443 | + | + | ++ | − | ++ | − | ++ | − |
| 1445 (B23) | ± | ± | − | − | ++ | ++ | ++ | ++ |
| 1440 (B25) | ++ | ++ | ++ | ± | ++ | ++ | ++ | ++ |
| 1442 (R23) | ± | − | − | − | ± | − | − | − |
| Rabbit sera | ||||||||
| 1 | − | − | − | ++ | − | − | − | − |
| 2 (R15) | ++ | ++ | + | − | ++ | + | + | + |
| 3 | ± | ++ | ± | − | − | ++ | − | − |
| 4 | ± | ++ | ++ | − | + | − | ++ | − |
| 5 | − | − | − | − | − | − | − | ++ |
a
Mice and rabbits were immunized with peptides coupled to KLH. Antisera diluted 1/200 were tested by dot spot assay against peptides coupled to BSA. No reaction was found with sera from nonimmunized animals. All antipeptide sera were reactive with the enzyme either in dot spot assays or ELISAs. Peptides were modelled on reactive sequences of the enzyme or on phage-displayed mimotopes (shown in parentheses). For peptide sequences and locations, see the text and Fig. 2. For reactivity symbols, see Fig. 1.