TABLE 1.
Strain or plasmid | Genotype or relevant characteristics | Reference or source |
---|---|---|
Strains | ||
E. coli DH5 | F−(Φ80dlacZΔM15) Δ(lacZY AargF)U169 deoR recA1 endA1 hsdR17 (rK− mK+), supE44 λ-thi-1 gyrA96 relA1 λ-CH616 | Gibco-BRL (7) |
B. quintana | ‘Oklahoma’ | |
Plasmids | ||
pBBR1-MCS2 | Kmr Mob Rep origin, Bartonella shuttle vector | Michael Kovach (7) |
pFPV25 | Source of promoterless GFP (gfpmut3a) | Raphael Valdivia |
PJMBGFP | PBBR1-MCS2 with a 970-bp HindIII fragment containing B. bacilliformis flagellin promoter (fla-pro) upstream of gfpmut3a | James M. Battitsti (2) |