KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal Anti-GAPDH	EMD Millipore	Cat# MAB374
Rabbit monoclonal Anti-HRAS	Proteintech	Cat# 18295-1-AP
Mouse monoclonal Anti-KRAS	Sigma	Cat# WH0003845M1
Rabbit monoclonal Anti-MYC	Cell Signaling Technologies	Cat# 5605S
Mouse monoclonal Anti-NRAS	Santa Cruz Biotechnologies	Cat# sc-31
Rabbit monoclonal Anti-phospho-AKT S473	Cell Signaling Technologies	Cat# 4060S
Rabbit monoclonal Anti-phospho-EGFR Y1068	Abcam	Cat# ab5644
Rabbit monoclonal Anti-phospho
ERK1/2 T202/Y204	Cell Signaling Technologies	Cat#4370S
Rabbit monoclonal Anti-phospho-FGFR3 Y724	Abcam	Cat#ab155960
Rabbit monoclonal Anti-phospho-HER2 Y1248	Cell Signaling Technologies	Cat# 2247S
Rabbit monoclonal Anti-phospho-MEK S217/S221	Cell Signaling Technologies	Cat#9154S
Rabbit monoclonal Anti-phospho-RSK1 S363/T359	Abcam	Cat#ab32413
Rabbit monoclonal Anti-phospho-SHP2 Y542	Cell Signaling Technologies	Cat#3751S
Amersham ECL Mouse IgG,
HRP-linked whole Ab (from sheep)	GE Life Sciences	Cat# NA931-1ML
Amersham ECL Rabbit IgG,
HRP-linked whole Ab	GE Life Sciences	Cat# NA934-1ML
Biological samples
Patient Derived Xenografts (PDX)	University of Texas MD Anderson Cancer Center	https://www.pdxnetwork.org/md-anderson
Chemicals, peptides, and recombinant proteins
AMG 510 (KRASG12C inhibitor)	MedChemExpress	Cat# HY-114277
ARS-1620 (KRASG12C inhibitor)	Sellechckem	Cat# S8707
BGJ398 (FGFR inhibitor)	Chemietek	Cat# CT-BGJ398
MRTX849 (KRASG12C inhibitor)	MedChemExpress	Cat# HY-130149
Panitumumab (Vectabix, EGFR mAb)	McKesson	Cat #765995
RM-018 (KRASG12C inhibitor)	Revolution Medicines	N/A
RMC-4450 (SHP2 inhibitor, in vitro)	Selleckchem	S8718
RMC-4550 (SHP2 inhibitor, in vivo)	Revolution Medicines	N/A
Trametinib (MEK inhibitor)	Selleckchem	Cat# S2673
Critical commercial assays
Active Ras detection kit	Cell Signaling Technologies	Cat# 8821
Human Phospho-Receptor
Tyrosine Kinase Array Kit	R&D systems	Cat # ARY001B
Lipofectamine RNAiMAX	Life Technologies	Cat# 13778100
Thermo ScientificTM SuperSignalTM West
Femto Chemiluminescent Substrate	Thermo Fisher	Cat# PI34096
Experimental models: Cell lines
Human B8182 colorectal adenocarcinoma	MD Anderson cell line bank	Katsiampoura et al. (2017)(1)
Human F3008 colorectal adenocarcinoma	MD Anderson cell line bank	Katsiampoura et al. (2017)(1)
Human LIM-2099 colorectal adenocarcinoma	Millipore Sigma	Cat# 12062002-1VL
Human MGH-1088 lung adenocarcinoma	Center for Molecular Therapeutics at the MGH Cancer Center	Crystal et al., 2014 (2)
Human MGH-1062 lung adenocarcinoma	Center for Molecular Therapeutics at the MGH Cancer Center	Crystal et al., 2014 (2)
Human MIA PaCa-2 pancreatic adenocarcinoma	ATCC	Cat# CRL-1420
Human NCI-H358 lung adenocarcinoma	ATCC	Cat# CRL-5807
Human SW837 colorectal adenocarcinoma	Center for Molecular Therapeutics at the MGH Cancer Center	ATCC, Cat# CCL-235
Human SW1463 colorectal adenocarcinoma	ATCC	Cat# CCL-234
Experimental models: Organisms/strains
Mouse, Nude, Crl:NU(NCr)-Foxn1nu, 6-8 wk female	Charles River Laboratories	N/A
Mouse, NOD/SCID, NOD.CB17-Prkdcscid/NCrCrl 8-week old female mice	Charles River Laboratories	N/A
Oligonucleotides
ON-Target Plus SMART pool Human HRAS	Dharmacon	L-003919-00-0005
ON-Target Plus SMART pool Human NRAS	Dharmacon	L-004142-00-0005
On-Target Plus Control Pool	Dharmacon	D-001810-10-05
Other
RPPA Arrays	MDA Anderson RPPA core facility	https://www.mdanderson.org/research/research-resources/core-facilities/functional-proteomics-rppa-core.html
Software and algorithms
GeneSys Acquisition Software, Version 1.4.1.1	Syngene	N/A
GraphPad Prism 6 Software	GraphPad	N/A
ImageJ	NIH	N/A
MORPHEUS software	Broad Institute	https://software.broadinstitute.org/morpheus/