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Sorting of Ear1 and Kex2 appears unaffected by Atg18, Atg21 and Hsv2. Ear1 (A) and Kex2 (B) were C-terminally tagged with GFP and expressed from the chromosome in either wildtype, the indicated deletion strains or, in case of Ear1, with overexpressed Atg18. FM 4–64 was used to stain the vacuolar membrane. Cells with increased GFP signal inside the vacuole were counted and the ratio of cells with stained vacuole to total amount of cells was calculated. Scale bar: 5 µm.
