Table 1.
Identification of Atg18 interactors with the BioID approach.
Gene | Replicate | Intensity |
Ratio |
Enrichment |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
light (BirA*-Atg18) |
medium (BirA*) |
heavy (empty) |
light/ medium |
light/ heavy |
light/ medium |
light/ heavy |
||||||
Atg18 | 1 | 2,783,100,000 | 225,250,000 | 99,951,000 | 12.36 | 12 | 27.84 | 79 | 92% | 91% | 96% | 97% |
2 | 4,149,700,000 | 464,070,000 | 212,590,000 | 8.94 | 19.52 | 89% | 95% | |||||
3 | 2,996,400,000 | 191,760,000 | 15,710,000 | 15.63 | 190.73 | 94% | 99% | |||||
Vac14 | 1 | 19,347,000 | 480,700 | 751,380 | 40.25 | 32 | 25.75 | 100 | 98% | 94% | 96% | 98% |
2 | 22,695,000 | 464,110 | 95,253 | 48.90 | 238.26 | 98% | 100% | |||||
3 | 38,807,000 | 5,002,400 | 1,087,700 | 7.76 | 35.68 | 87% | 97% | |||||
Fab1 | 1 | - | - | - | n.a. (6) |
- | n.a. (156) |
- | 91% | - | 100% | |
2 | 982,570 | 0 | 0 | n.a. | n.a. | 100% | 100% | |||||
3 | 1,895,500 | 323,500 | 12,140 | 5.86 | 156.14 | 83% | 99% | |||||
Atg2 | 1 | 2,221,700 | 108,290 | 51,979 | 20.52 | n.a. (21) |
42.74 | n.a. (43) |
95% | 98% | 98% | 99% |
2 | 1,452,800 | 0 | 0 | n.a. | n.a. | 100% | 100% | |||||
3 | 1,966,800 | 0 | 0 | n.a. | n.a. | 100% | 100% | |||||
Vps35 | 1 | 4,604,000 | 790,540 | 96,105 | 5.82 | 9 | 47.91 | n.a. (48) |
83% | 88% | 98% | 99% |
2 | 14,455,000 | 1,298,800 | 0 | 11.13 | n.a. | 91% | 100% | |||||
3 | 9,986,000 | 906,900 | 0 | 11.01 | n.a. | 91% | 100% | |||||
Snx3 | 1 | 1,411,000 | 0 | 0 | n.a. | n.a. (6) |
n.a. | n.a. (76) |
100% | 85% | 100% | 100% |
2 | 1,844,400 | 611,410 | 24,183 | 3.02 | 76.27 | 67% | 99% | |||||
3 | 2,603,300 | 320,970 | 0 | 8.11 | n.a. | 88% | 100% |