Table 2.
Strains used.
| strain | genotype | reference |
|---|---|---|
| BY atg18∆ vps17∆ Vps35-6xHA | atg18∆::hphNT1 vps17∆::KAN VPS35-6xHA::NatNT2 | This study |
| BY atg18∆ vps26∆ Vps35-6xHA | atg18∆::hphNT1 vps26∆::KAN VPS35-6xHA::NatNT2 | This study |
| BY atg18∆ vps29∆ Vps35-6xHA | atg18∆::hphNT1 vps29∆::KAN VPS35-6xHA::NatNT2 | This study |
| BY atg18∆ vps5∆ | atg18∆::KAN vps5∆::natNT2 | This study |
| BY atg18∆ vps5∆ Vps35-6xHA | atg18∆::hphNT1 vps5∆::KAN VPS35-6xHA::NatNT2 | This study |
| BY PGPD1-Vps5 | natNT2::PGPD1-VPS5 | |
| BY vps35∆ atg27∆ | vps35∆::hphNT1 atg27∆::KAN | This study |
| WCG atg18∆ arg4∆ lys1∆ | atg18∆::KAN arg4∆::hphNT1 lys1∆::natNT2 | This study |
| WCG atg18∆ Atg2-3xHA | atg18∆::KAN ATG2-3xHA::hphNT1 | This study |
| WCG atg18∆ atg21∆ hsv2∆ Ear1-yeGFP | atg18∆::KAN atg21∆::natNT2 hsv2∆::His3 Ear1-yeGFP::hphNT1 | This study |
| WCG atg18∆ atg21∆ hsv2∆ Kex2-GFP | atg18∆::KAN atg21∆::natNT2 hsv2∆::His3 Kex2-yeGFP::hphNT1 | This study |
| WCG atg18∆ Snx3-6xHA | atg18∆::KAN SNX3-6xHA::natNT2 | This study |
| WCG atg18∆ Vac14-6xHA | atg18∆::KAN VAC14-6xHA::natNT2 | This study |
| WCG atg18∆ Vps26-6xHA | atg18∆::KAN VPS26-6xHA::hphNT1 | This study |
| WCG atg18∆ Vps29-6xHA | atg18∆::KAN VPS29-6xHA::hphNT1 | This study |
| WCG atg18∆ vps30∆ Vps35-6xHA | atg18∆::KAN vps30∆::hphNT1 Vps35-6xHA::natNT2 | This study |
| WCG atg18∆ Vps35-6xHA | atg18∆::KAN VPS35-6xHA::NatNT2 | This study |
| WCG atg18∆ Vps35-6xHA PGPD1-Vps5 | atg18∆::KAN VPS35-6xHA::hphNT1 natNT2::PGPD1-VPS5 | This study |
| WCG atg18∆ Vps35-6xHA Vps5-GFP | atg18∆::KAN VPS35-6xHA::natNT2 VPS5-yeGFP::hphNT1 | This study |
| WCG atg18∆ Vps35-mCherry | atg18∆::KAN VPS35-mCherry::hphNT1 | This study |
| WCG atg18∆ vps35∆ | atg18∆::KAN vps35∆::hphNT1 | This study |
| WCG atg18∆ vps35∆ Vps26-6xHA | atg18∆::KAN vps35∆::His3 VPS26-6xHA::hphNT1 | This study |
| WCG atg18∆ vps5∆ Vps35-6xHA | atg18∆::hphNT1 vps5∆::KAN VPS35-6xHA::natNT2 | This study |
| WCG atg2∆ atg9∆ atg18∆ Vps35-6xHA | atg2∆::hphNT1 atg9∆::loxP atg18∆::KAN VPS35-6xHA::natNT2 | This study |
| WCG Atg9-GFP | ATG9-yeGFP::hphNT1 | This study |
| WCG Ear1-GFP | EAR1-yeGFP::hphNT1 | This study |
| WCG Ear1-GFP PGPD1-Atg18 | Ear1-GFP::hphNT1 natNT2::PGPD1-ATG18 | This study |
| WCG Kex2-GFP | KEX2-yeGFP::hphNT1 | This study |
| WCG Vps35-6xHA Vps5-GFP | VPS35-6xHA::natNT2 VPS5-yeGFP::hphNT1 | This study |
| WCG Vps35-6xHA Vps5-GFP PGPD1-Atg18 | VPS35-6xHA::natNT2 VPS5-yeGFP::hphNT1 KAN::PGPD1-ATG18 | This study |
| WCG vps35∆ Atg9-GFP | vps35∆::His3 ATG9-yeGFP::hphNT1 | This study |
| WCG vps35∆ Ear1-GFP | vps35∆::His3 EAR1-yeGFP::hphNT1 | This study |
| WCG vps35∆ Kex2-GFP | vps35∆::His3 KEX2-yeGFP::hphNT1 | This study |
| WCG vps5∆ Atg9-GFP | vps5∆::natNT2 Atg9-yeGFP::hphNT1 |