Table 3.
Plasmids used.
| Plasmid | genotype | reference |
|---|---|---|
| GFP-Atg18 | pUG36 PMET17-GFP-ATG18-TCYC1 | [22] |
| GFP-Atg18FTTG | pUG36 PMET17-GFP-ATG18FTTG-TCYC1 | [22] |
| MYC-BirA*-Atg18 | pUG36 PMET17-MYC-BirA*-ATG18-TCYC1 | This study |
| MYC-BirA* | pUG36 PMET17-MYC-BirA*-TCYC1 | This study |
| GFP-Atg8 | pRS316 PATG8-GFP-ATG8-TATG8 | [55] |
| Pgk1-GFP | pRS316 PPGK1-PGK1-GFP-TADH1 | [36] |
| Atg2-GFP | pUG23 PMET17-ATG2-GFP-TCYC1 | This study |
| GFP-Atg9 | PMET17-GFP-ATG9-TCYC1 | [56] |
| Atg18-GFP | PATG18-ATG18-GFP-TCYC1 | This study |
| Vps35-6xHA | PVps35-VPS35-6xHA-TCYC1 | This study |
| Vps35∆6-6xHA | PVps35-VPS35∆6-6xHA-TCYC1 | This study |
| Vps35-Cub-RURA | PMET17-VPS35-Cub-RURA3 | This study |