Skip to main content
. 2022 May 18;19(1):256–277. doi: 10.1080/15548627.2022.2072054

Figure 3.

Figure 3.

Tubular autophagy deficiency in iRT-atg7-KO mice suppresses senescent changes in renal tubules during post-ischemic kidney repair. WT and iRT-atg7-KO mice underwent sham operation or 30-min unilateral renal ischemia followed by reperfusion for up to 4 weeks. Left kidneys were harvested at the indicated time points for senescence analysis. (A) RT-qPCR of Cdkn2a/p16 mRNA (n = 5 or 6 for each group). (B) SA-GLB1/β-gal staining. Scale bar: 50 µm. (C) Quantification of SA-GLB1/β-gal-positive stained areas (UI30R 2 w: WT n = 5, KO n = 8; UI30R 4 w: WT n = 5, KO n = 8). (D) γ-H2AX and MKI67 co-immunofluorescence. Scale bar: 20 µm. White arrows indicate typical senescent tubular cells with 4 or more γ-H2AX-positive foci and MKI67-negative. (E) Quantification of the percentage of senescent tubular cells (n = 5 or 6 for each group). Data in (A, C and E) are presented as mean ± SD. Two-way ANOVA with multiple comparisons was used for statistics.