Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 27;20(1):20–30. doi: 10.1080/15476286.2022.2159158

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — In vitro RNAi in FsK-sGFP. (A) Schematic representation of the sGFP transgene that is present in FsK-sGFP. PToxA: promoter for the promoter from Pyrenophora tritici-repentis ToxA gene; sGFP: GFP variant that contains a serine-to-threonine substitution at amino acid 65; TNOS: terminator for the nopaline synthase gene. The 445 bp fragment chosen for in vitro transcription of dsRNA is depicted. (B) Stereoscopic observation of sGFP fluorescence. (C) Fluorometric analysis for in vitro RNAi in FsK-sGFP. Vertical axis: RFU: relative fluorescence unit, calculated as the ratio of sGFP-indicative fluorescence (excitation 488 nm, emission 515 nm) to growth-indicative absorbance (wavelength 595 nm). Horizontal axis: 1–12: 12 wells containing FsK-sGFP conidia. 13–24: 12 wells containing FsK-sGFP conidia plus 100 ng (each well) sGFP dsRNA.