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. 2022 Apr 25;19(1):163–179. doi: 10.1080/15548627.2022.2063005

Figure 7.

Figure 7.

Elevated citrate levels in mammalian follicular fluid promote oocyte maturation. (A) A schematic showing the procedure for human follicular fluid (hFF) isolation. (B) The negative correlation of citric acid levels with age in hFF. n = 42. (C) The porcine follicular fluid (pFF) citric acid levels in different sizes of porcine follicles. n = 27. D indicated diameter of follicle. Data are expressed as mean ±SEM. ***p < 0.001. (D) The positive correlation of citric acid levels with porcine oocyte maturation. n = 27. Correlation and corresponding statistical significance were calculated based on Spearman’s rank correlation coefficient (R) in R language. (E) Representative images of porcine oocytes in vitro maturation. (F) Average polar body rate of porcine oocytes in control (n = 6) and citrate treated groups (n = 6). Data are expressed as mean ±SEM. ***p < 0.001. The lines in (b) and (d) indicates the 0.95 confidence interval around smooth. ***p < 0.001 by unpaired two-tailed Student’s t test with R language.