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. Author manuscript; available in PMC: 2023 Jan 3.
Published in final edited form as: Biochemistry. 2022 Sep 7;61(18):1955–1965. doi: 10.1021/acs.biochem.2c00378

Figure 3.

Figure 3.

ITC data for CaM binding to CaM1 and CaM2. ITC isotherms of CaM1 binding to (A) full-length CaM, (B) CaM N-lobe, and (C) CaM C-lobe. ITC isotherms of CaM2 binding to (D) full-length CaM, (E) CaM N-lobe, and (F) CaM C-lobe. Each isotherm was fit to either a one-site (in panels B, C, and F) or two-site model (in panels A, D and E), and the binding parameters (ΔH and Kd) are given in Table 1. The CaM1 or CaM2 peptide concentrations were each 50 μM in 1.5 mL in the sample cell for titration with 0.5 mM CaM (or lobes) using 34 injections of 7 μL each.