Table 1.
ITC Binding Parameters
| sample | K1 (M−1) | ΔH1 (kcal/mol) | K2 (M−1) | ΔH2 (kcal/mol) |
|---|---|---|---|---|
| CaM/CaM1 | 1.1 ± 0.1 × 106 | −7.5 ± 0.2 | 4.2 ± 0.5 × 103 | +40 ± 4 |
| CaM C-lobe/CaM1 | 3.4 ± 0.7 × 104 | +2.0 ± 0.4 | ||
| CaM N-lobe/CaM1 | 1.8 ± 0.2 × 105 | −2.5 ± 0.2 | ||
| CaM/CaM2 | 7.3 ± 1.0 × 106 | −4.5 ± 1.6 | 2.9 ± 0.5 × 106 | +16 ± 2 |
| CaM C-lobe/CaM2 | 9.8 ± 1.0 × 106 | −2.0 ± 0.1 | ||
| CaM N-lobe/CaM2 | <1.8 × 104 | +7.5 ± 4.0 | ||
| CaM N-lobe/CaM1F575E | <2.0 × 104 | +0.7 ± 0.5 | ||
| CaM C-lobe/CaM2L1132E | 1.8 ± 0.5 × 104 | +3.1 ± 0.5 | ||
| CaM C-lobe/CaM2L1136E | 1.2 ± 0.7 × 104 | +2.6 ± 1.8 |