TABLE 1.

Take parts as an example: Gene manipulation promotes exosome release.

Modification	Cell line	Outcome	Mechanism of action	References
Overexpression of HSP20	Cardiomyocyte	Increased generation of exosomes	Direct interaction of Hsp20 with Tsg101	Wang et al. (2016)
Overexpression of LMP1	HEK293	Separation EV increased 2–4 times	CD63-mediated exosomes LMP1 release	Hurwitz et al. (2017)
Overexpression of TSPAN6	HEK293	Exosome release increased by 60%	Interaction with syntenic	Guix et al. (2017)
Cortactin overexpression	SCC61	Exosome secretion increased 1.5–2 times	Interaction with Arp2/3 complex and F-actin	Sinha et al. (2016)
Overexpression of the tetraspanin CD9	Standard HEK-AAV producer	The production of exosomes increased 3.75 times	Not mentioned	Schiller et al. (2018)
Combined expression of STEAP3, syndecan-4	HEK293	The production of exosomes increased 15–40 times	Not mentioned	Kojima et al. (2018)
miR-126-3p-overexpressing combined with chitosan wound dressing	SMSCs	Increased exosome release	Not mentioned	Tao et al. (2017)