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PCR amplicon restriction fragment analysis. Fragments resulting from digestion of region 1 amplicons with Sau3A (A) or digestion of region 2 amplicons with BstXI (B) were resolved by agarose gel electrophoresis and visualized by staining with ethidium bromide. (A) Lanes: 1, MBORD731; 2, MBORD831; 3, MBORD901; 4, St. Louis; 5, 5107. (B) Lanes: 1, MBORD676; 2, MBORD606; 3, ATCC 19395; 4, MBORD626; 5, MBORD591. The locations of molecular size markers are indicated on the left.
