Figure 1.

Mutational Analysis in Exon 2 of the DPYD Gene. (a) 2% agarose gel electrophoresis of PCR amplified exon 2 (370 bp). Lanes from 1-12 are representative CRC patient’s samples, lane 13 represents negative control and lane M represents 100 bp DNA ladder. (b) Representative partial chromatogram for exon 2 showing rs1801265 (c.85T>C) variant. Wild type sequence (TGCGT)in the upper inset and homozygous variant sequence (TGTGT) in the lower in set. (c) Representative partial chromatogram for exon 2 showing wild type sequence (TGCGT) in the upper inset and heterozygous (c.85T>C) variant sequence (TGYGT) in the lower inset