Figure 2.

Mutational Analysis in Exon 13, Intron 13 and Exon-Intron Boundaries of the DPYD Gene. (a) 2% agarose gel electrophoresis of PCR-amplified product (409 bp). Lanes from 1-11 are representative CRC patient’s samples, lane 12 represents negative control and lane M represents 100 bp DNA ladder. (b) Representative partial chromatogram showing rs2811178(c.1740+40A>G) variant. Wild type sequence (TCATA) in the upper inset and homozygous variant sequence (TCGTA) in the lower inset. (c) Representative partial chromatogram showing wild type sequence (TCATA) in the upper inset and heterozygous (c.85T>C) variant sequence (TCRTA) in the low inset