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Mutational Analysis in Exon 13, Intron 13 and Exon-Intron Boundaries of the DPYD Gene. (a) 2% agarose gel electrophoresis of PCR amplified product (409 bp). Lanes from 1-11 are representative CRC patient’s samples, lane 12 represents negative control and lane M represents 100 bp DNA ladder. (b) Representative partial chromatogram showing (g.97515583_97515584insA) variant. Wild type sequence (TCTTT) in the upper inset and insertion variant sequence (TCTTA) in the lower in set
