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. 2022 Sep;23(9):2953–2964. doi: 10.31557/APJCP.2022.23.9.2953

Table 1.

Cycling Condition for RT-PCR

Cycle Temperature (°C) Duration
Initial activation 1 95 5 minutes
35 95 10 seconds
60 30 seconds
Dissociation 1 95 15 seconds
60 20 seconds
95 15 seconds