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. Author manuscript; available in PMC: 2023 Jan 3.
Published in final edited form as: Sci Signal. 2022 Sep 27;15(753):eabk1147. doi: 10.1126/scisignal.abk1147

Figure 5. SCA14 mutants are more rapidly turned over in the presence of cycloheximide.

Figure 5.

(A and B) Western blot analysis of lysates from COS7 cells transfected with HA-tagged WT PKCγ, PKCγ lacking the C1B domain (ΔC1B), PKCγ lacking the C1A domain (ΔC1A), or the indicated SCA14 mutants. COS7 cells were treated with CHX (355 μM) for 0, 6, 24, or 48 hours prior to lysis. Membranes were probed for HA (PKCγ) as well as endogenous expression of vinculin as a loading control. *, phosphorylated species; -, unphosphorylated species. Blot is representative (A) with quantification of total levels of PKCγ at each time point from three independent experiments shown in (B) as a percentage of initial level of PKC (0 hours) and represents mean ± S.E.M. Points were curve fit by non-linear regression.