FIG. 1.

Influence of RfaH on chuA expression of E. coli strain 536. (A) Detection of ChuA levels by Western blot analysis of whole-cell extracts of E. coli strain 536 and its derivatives using a HemR-specific antiserum. The strains were grown in the presence (+) or absence (−) of 0.4 mM 2,2′-dipyridyl. (B) Analysis of chuA transcript levels of E. coli strain 536 and its derivatives. An enhanced chemiluminescence-labeled chuA-specific probe was hybridized to total RNA isolated from strain 536 (lane 1), 536rfaH::cat (lane 2), 536rfaH::cat (pSMK1) (lane 3). The 23S and 16S rRNA were stained with 0.3% methylene blue after transfer of separated total RNA to a nylon membrane (internal control).