Table 3.

Antigenic epitopes and aa surface distribution of each unique mutation sites

	Amino acid residue$	Exposure on the surface*	Located within B cell Epitope#
VP1	4 N	√	√
	45S
	55D
	60 K	√
	73F
	76I
	103 N	√	√
	104 V	√	√
	105D	√	√
	114 L
	126S
	131A	√	√
	132D		√
	133S	√	√
	182S	√	√
	210 V	√
	214 T
	234F	√
	242A
	245P		√
	248D
	251 K	√	√
	256 T	√	√
	257E	√	√
VP2	134F	√	√
	160D	√	√
	162R	√	√
	170S	√	√
	177F	√	√
	180E	√	√
	251 V	√	√
	268I		√

$: The number of Amino acid residue is based on the VP1 or VP2 amino acid sequence of PTV HeNZ1. The amino acid residues listed in this table are the unique mutation sites identified in multiple sequence alignment, which were shown in Fig. 3A and Fig. 4A. a: spDBV 4.10was used to determine the amino acid residues exposed on the protein surface. #: B cell epitopes of VP1 and VP2 were predicted by using Disco Tope (http://www.cbs.dtu.dk/services/DiscoTope/) and displayed in Fig. 3A and Fig. 4A