Extended Data Fig. 3. Decreased thymic NKT cells in Nir3^–/– mice.

(a) Representative staining of NKT cells gated as TCRβ^int, CD1d-PBS-57 tetramer+ cells in live, CD8-negative thymocytes from WT and Nir3^–/– thymus. (b) Absolute numbers of thymic NKT cells in the WT and Nir3^–/– mice. (c) NKT cell subsets were gated as PLZF^lo RORγt^- (NKT1), PLZF^hi RORγt^- (NKT2) and PLZF^int RORγt⁺ (NKT17). (d) PLZF expression levels in total WT and Nir3^–/– NKT cells. (e-g) Absolute numbers of the NKT cell subsets in WT and Nir3^–/– mice. N = 5 mice. Data are shown as the mean ± s.e.m. and are representative of two independent experiments. P values were determined using a two-tailed Student’s t-test.

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