Fig. 4. Positive selection is modulated by Nir3.
a, Positive selection was visualized by TCRβ and CD5 in Nir3–/– and WT DP thymocytes. b,c, BM cells from 6- to 8-week-old CD45.1/CD45.2 Nir3 sufficient female mice and CD45.2 Nir3–/– female mice were mixed at 1:1 ratio and adoptively transferred into 8-week-old CD45.1 female hosts. Six weeks after the transfer, WT and Nir3–/– thymocytes at each development stage were identified by congenic CD45.1 and CD45.2 markers (b). The percentages of WT cells and Nir3–/– cells in each stage were quantified (c). d, Number of total thymocytes in WT and Nir3–/– OT-I mice were quantified. e–g, Representative flow plots (e) and quantification (f,g) of CD4 versus CD8 profiles of thymocytes from 8-week-old Nir3–/– OT-I and WT OT-I female mice are shown. h,i, Positive selection was visualized by TCRβ and CD5 expression on Nir3–/– OT-I and WT OT-I DP thymocytes (h) and quantified (i). n = 4 mice. Data are shown as the mean ± s.e.m. and are representative of two or three independent experiments. P values were determined using an unpaired, two-tailed Student’s t-test.