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. 2022 Dec 29;24(1):136–147. doi: 10.1038/s41590-022-01372-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — WT or Nir3^–/– DP thymocytes loaded with the calcium sensitive dye Indo-1 AM were incubated with indicated amount of anti-CD3 antibody at time 0 and stimulated with crosslinking antibodies, thapsigargin or ionomycin at the arrow. The [Ca²⁺]_I elevation of each DP subset, as gated in Fig. 4a, was assessed based on the Indo-1 fluorescence emission ratio 405nm/485nm by flow cytometry. n > 5 cells. All data are shown as the mean ± s.e.m. and are representative of at least two independent experiments.

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