Extended Data Fig. 8. NLRP3 activators do not affect overall TGN integrity.

a, Confocal images of HeLa cells treated with 10 μM nigericin for indicated times. Cells were co-stained with antibodies against EEA1 and Golgin97. DAPI was used to stain the nucleus. Scale bar: 10 μm. b, Pearson’s correlation coefficient of fluorescence of EEA1 and Golgin97 in experiments shown in panel a. Means ± SD, N = 3, n = 23 cells for ‘0 min’, n = 25 cells for ‘20 min’ and n = 36 cells for ‘40 min’ group. c, Confocal images of HeLa cells treated with 45 μg ml⁻¹ CL097 for indicated times. Cells were co-stained with antibodies against GCC2 and p230. DAPI was used to stain the nuclear. Scale bar: 10 μm. d, Pearson’s correlation coefficient of fluorescence of GCC2 and p230 in experiments shown in panel c. Means ± SD, N = 3, n = 16 cells for ‘0 min’, n = 16 cells for ‘40 min’ and n = 19 cells for ‘80 min’ group. e, Confocal images of HeLa cells treated with 45 μg ml⁻¹ CL097 for indicated time points. Cells were co-stained with antibodies against GCC1 and Golgin97. DAPI was used to stain the nuclear. Scale bar: 10 μm. f, Pearson’s correlation coefficient of fluorescence of GCC1 and Golgin97 in experiments shown in panel d. Means ± SD, N = 3, n = 16 cells for ‘0 min’, n = 17 cells for ‘40 min’ and n = 19 cells for ‘80 min’ group. Data were analyzed with an unpaired two-sided t-test (b, d, f). Data shown in a, c, e are representative of at least three independent experiments.