Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 23;24(1):174–185. doi: 10.1038/s41590-022-01366-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — (a-d) Analysis of TCR repertoires in CD4⁺ and CD8⁺ LN T cells, and mature SP4 and SP8 thymocytes from Lck^WT/WT (gray), Lck^CA/CA (blue) and Lck^CA/KR (red) mice. The information about the sample size are in Supplementary Table 6. Individual mice and means are shown. Related to Fig. 2e-g. (a) The usage of TRAV and TRBV gene segments in the indicated mice. Each bar represents the average frequency of the gene segment among all CDR3 sequences in a particular group of samples. (b) A total percentage of typical TCRβ (TRB) gene segments used by NKT cells (TRBV1,TRBV13-2, and TRBV9) in the indicated samples from the indicated mice. (c) Percentage of TCRs containing TRAV11-TRAJ18 gene segments typically used by NKT cells in the indicated cells from the indicated mice. (d) Diversity of the repertoire of the TCRα (TRA) and TCRβ (TRB) CDR3 amino acid sequences in the indicated cells from the indicated mice calculated using the Chao1 richness estimator. CD4 and CD8 T-cell CDR3 amino acid sequences were analyzed together (left) or separately (right). Invariant NKT TRAV11-TRAJ18 CDR3 amino acid sequences were removed before this analysis. The statistical significance was calculated using a Kruskal-Wallis test with multiple comparison adjustment using the Holm method.

Source data