Extended Data Fig. 3. Phagocytes from Shp2^f/fLysM^Cre and Shp2^f/f mice have distinct metabolic activities.

a, Phagocytes were generated from primary bone marrow cells of Shp2^f/fLysM^Cre and Shp2^f/f mice using GM-CSF and metabolite analysis was performed after 48 hours of culture. Principal component analysis (PCA). b, Unsupervised hierarchical clustering of top 75 metabolites (log2FC ≥ 1). c, Individual graphs of relative peak intensity of representative intermediate metabolites of glycolysis, PPP and TCA cycle. Results from one representative of two independent experiments are shown. The amounts of the indicated metabolites were plotted in whisker boxes. The lower and upper sides of the box indicate the first and third quartile, respectively. The horizontal line inside the box indicates the median value, whereas the lower and upper bars indicate the minimum and maximum of distribution, respectively.