Extended Data Fig. 4. Myeloid cells of Shp2^f/fLysM^Cre mice have distinct molecular and functional properties.

a, GO Biological Processes Pathways enriched among top 500 upregulated genes in TAMs from Shp2^f/fLysM^Cre MC17-51 tumor-bearing mice compared to Shp2^f/f MC17-51 tumor-bearing mice, collected at day 15 after tumor implantation. Differential gene expression analysis was performed using DESeq2 and ClusterProfiler (v3.12.0) was utilized for downstream functional investigations. b, c, Shp2^f/f and Shp2^f/fLysM^Cre mice injected with MC17-51 fibrosarcoma were treated with either anti-CD3 antibody or control IgG at day -1 relative to tumor injection and subsequently every third day, and tumor growth was monitored for 12 days (b). Results show means of tumor volume and are representative of one from two independent experiments with n = 10 mice per group, (***p < 0.001) unpaired t-test two tailed. c, At termination, expression of lymph node CD4⁺ and CD8⁺ T cells was assessed by flow cytometry. One representative histoplot of each treatment condition generated from Shp2^f/fLysM^Cre mice is shown. (In this experiment, the number of injected MC17-51 cells was reduced by 50% because, after T cell depletion, tumors in Shp2^f/f mice rapidly exceeded the permitted size).