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. 2023 Jan 3;14:1. doi: 10.1038/s41467-022-34464-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Clustering analysis of single-cell transcriptome profiles of E9.5 mouse foregut cells. b Isl1 expression projected on clusters in (a). Dashed lines delineate the splanchnic mesenchyme cluster. c Lateral view of an E9.5 mouse embryo. Dashed line indicates a transverse section at the pancreatic level. d Immunostaining on mouse foregut indicated in (c). n = 3 embryos. e, f Whole mount immunostaining of a dissected pancreas. Yellow dashed lines delineate the epithelium, while the yellow and the white lines delineate the splanchnic mesenchyme. n = 3 pancreata. g Schematic of the I^creT mouse model. h–k Co-immunostaining of the adult pancreas and quantification of cells that are either single positive or double positive for defined markers. n = 5 mice. l–n Flow cytometry analysis of I^creT adult pancreas and quantification of Tomato+ cells within populations defined by markers. Tomato-, n = 5 mice; I^creT, n = 9 mice. Data are mean ± SD. Two-way ANOVA test with Tukey’s multiple comparisons was performed. ** indicates p < 0.0001. ns, not significant, p = 0.547. E, embryonic day; epi, epithelium; meso, mesoderm; spl, splanchnic mesenchyme. Arrowheads indicate Tomato+ cells. Scale bars in (c–f): 100 μm; in (h–j): 30 μm. Source data are provided as a Source data file.