Fig. 3. The majority of pancreatic cancer-associated fibroblasts are targeted in the KPFI^creT model.

a Schematic of the KPFI^creT mouse model. b, c Bright field and red epifluorescence views of dissected pancreata and spleens. Normal, n = 3 mice; PanIN, n = 6 mice; PDAC, n = 3 mice. Arrowhead denotes a puncta of red fluorescence. d–f Co-immunostaining on pancreatic tissues and quantification of cells that are either single positive or double positive for defined markers. PanIN, n = 4 mice; PDAC, n = 5 mice. g–I Co-immunostaining on pancreatic tissues and quantification of cells that are either single positive or double positive for defined markers. PDGFRβ, n = 5 mice; αSMA, n = 4 mice. j–l Flow cytometry analysis of a KPFI^creT pancreas and quantification of Tomato+ cells within populations defined by markers. Tomato-, n = 5 mice; KPFI^creT, n = 7 mice. Data are mean ± SD. Two-way ANOVA test with Tukey’s multiple comparisons was performed. **** indicates p < 0.0001; ns, not significant, p = 0.379. PanIN, pancreatic intra-epithelial neoplasia; PDAC, pancreatic ductal adenocarcinoma; spl, spleen; pan, pancreas. Arrowheads denote Tomato+ cells. Scale bars in (b, c): 1000 μm; in (d, e): 50 μm; in d’, e’, g, h: 30 μm. Source data are provided as a Source data file.