Fig. 4. KPFI^creERT model specifically targets fibroblasts in the pancreas.

a–h Co-immunostaining of pancreatic tissues (focusing on either the islet region or the acinar region) comparing the I^creT model and the I^creERT model treated with Tamoxifen at indicated times points. Arrowheads denote Tomato+ epithelial cells. i Quantification of cells that are either single positive or double positive for defined markers in images represented in (a–h). I^creT, n = 5 mice; I^creERT + Tam E8, n = 5 mice; I^creERT + Tam E9, n = 6 mice; I^creERT + Tam E10, n = 4 mice. j Schematic the KPFI^creERT mouse model. k, l Bright field and red epifluorescence views of dissected pancreata and spleens. I^creERT, n = 6 mice; KPFI^creERT, n = 7 mice. m Quantification of Tomato+ cells within PDPN+, PDGFRα+, CD45+, EpCAM+, or CD31+ cells identified by flow cytometry. Tomato-, n = 5 mice; I^creERT, n = 8 mice; KPFI^creERT, n = 7 mice. n–q Co-immunostaining of Tomato, VIM, and PDGFRβ on normal and PDAC pancreatic tissues. Arrowheads indicate double positive cells. Data are mean ± SD. Two-way ANOVA test with Tukey’s multiple comparisons was performed. **** indicates p < 0.0001; ns, not significant, p > 0.9999. Spl, spleen; pan, pancreas. Scale bars in a–h and n–q: 30 μm. Scale bars in k, l: 1000 μm. Source data are provided as a Source data file.