(A, B, C) Mapping of CENP-A-bound reads in CHM13 (blue), PD-NC4 (black), and HeLa cells (maroon) at the centromeres of chromosome X (A), chromosome 2 (B), and chromosome 17 (C). Dashed lines mark regions that are differentially enriched for CENP-A binding between cell lines. Annotation tracks indicate positions of centromeric satellites (CenSat), higher order repeat arrays (ASat HOR), rough HOR age based on evolutionary age of HOR-haps (HOR evolutionary age), and the location of the zone of recent HOR expansion (zone of recent expansion). HOR age and zone of recent expansion were adapted from (Altemose et al, 2022). Black lines in (A, B) indicate the active centromere size in Kb.