Fig. 2.

Increased expression of liver pro-inflammatory cytokines and TLRs in AIC mice. Male and female mice were immunized with 2-OA-OVA or normal saline and examined at 3 weeks post-immunization. The expression levels of (A) IFN-γ and TNF-α and (B) TLRs in the liver were detected using RT-qPCR. M, male mice; F, female mice. Symbols represent one individual mouse, and bars indicate mean ± SEM. n = 8–12 mice per group. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 (two-way ANOVA followed by Tukey's multiple comparison test).