Fig. 1.

Detection of differences in peptide potency using Jurkat lines Jurkat cells expressing a the TCR from the islet-infiltrating CD4⁺ T-cell clone, A3.10. For all panels the blue circles are full-length WT C-peptide (PI33-63), the teal triangles short C-peptide (PI38-54) and the pink hexagons are responses to HIP (Cpept-IAPP2 HIP) known to be a potent stimulator of this clone (see Supplementary Table 4 for sequences). (A) IL-2- peptide dose response curve with a CD4⁺ Jurkat cells expressing the A3.10 TCR. Mean of triplicate measure and bars are the SEM. (B) shows the inverse of the EC50 for the titration shown in panel A. Unpaired t-test (** = p < 0.01). (C) Fold change in EC50 relative to full-length C-peptide (PI33-63). For panels B and C each point represents an independent experiment.