Skip to main content
. 2022 Mar 2;43:219–231. doi: 10.1016/j.jare.2022.02.015

Fig. 4.

Fig. 4

Effect of crocin on the differentiation and proliferation of stem neural cell in vitro. (A) Immunofluorescence staining of primary NSC cells with Ki67 antibodies. Cells were treated with 2 μM or 5 μM crocin. Scale bar: 50 μm. (B) Immunofluorescence staining of primary NSC cells with NeuN, DCX, DAPI antibodies. Cells were treated with 2 μM or 5 μM crocin. Scale bar: 50 μm. (C) The representative pictures of primary NSCs in concentric sholl radii. ells were treated with 2 μM or 5 μM crocin. Scale bar: 10 μm. (D-E) Quantification of Ki67+ and NeuN+/DCX-/DAPI cells. (F) The dendritic length after differentiation in 2 μM crocin-treated, 5 μM crocin-treated or control group. (G) The intersection number after differentiation in 2 μM crocin-treated, 5 μM crocin-treated or control group. Results are presented as mean ± SEM. Statistical analysis: one-way ANOVA followed by Bonferroni post hoc test. **p < 0.01.