Fig. 7.

Crocin-related effect on depressive symptoms and neurogenesis were reversed by Dkk1 treatment. (A) Timeline of Dkk1 experiment. (B) Cannula position and Hoechst33342 dye infusions into the DG of mice. (C-D) Mice pretreated with Dkk1 followed by crocin treatment did not exhibit reduced immobility time in TST and FST. (E-F) Crocin significantly increased the number of Ki67 positive cells and number of mature neurons in Vehicle group, but not in Dkk1 group. (G-J) Effects of crocin treatment on Wnt3a, p-β-catenin and p-GSK3β protein levels in the hippocampus of Dkk1 mice. Results are presented as mean ± SEM. Statistical analysis: two-way ANOVA followed by Tukey post hoc test. *p < 0.05; **p < 0.01.